There are various microbial insect diseases that occur naturally and can be used as biological pesticides. These microbes outbreaks and make the insect populations denser. The bacteria used for biological control infect insects through their digestive tracts and hence it becomes difficult to control insects with sucking mouth parts like aphids and scale insects, with bacterial biological control. The most widely applied species of bacteria is Bacillus thuringiensis that is used for biological control, with four sub-species for controlling Lepidopteran, Dipteran and Coleopteran insect pests. The Fungi are also used for biological control and fungi that cause disease in insects are called as entomopathogenic fungi that include fourteen species of entomophthoraceous fungi which attack aphids. The species in the genus Trichoderma are also used for managing some soilborne plant pathogens.
There are various plants that are used for regulating insect pests. By selecting a wide range of plants for the garden, helps in regulating pests in a various ways such as masking the crop plants from pests as per the proximity of the companion, serving as nursery plants that provide breeding grounds for beneficial insects and producing olfactory inhibitors and odours for confusing and deterring pests. Other ways of using plants is acting as trap plants by offering an alluring food for enticing pests away from the crops.
The plants can also provide an alternative habitat in a form of a hedgerow, or beetle bank or shelterbelt where beneficial insects can reproduce and live. There are nectar-rich plants that bloom for longer periods as many beneficial are parasitic or predatory as larvae however, nectivorous during the adult stage. A good example of it is the soldier beetle, found on flowers as an adult, but its larvae feed on aphids, beetles, caterpillars, and grasshopper eggs. The countries like Benin and Vietnam use the legume vine Mucuna pruriens as a biological control for problematic Imperata cylindrica grass. The Mucuna pruriens is not invasive outside its cultivated area.